piston gradient fractionater Search Results


piston gradient fractionator  (Biocomp Instruments Inc)
86
Biocomp Instruments Inc piston gradient fractionator
Piston Gradient Fractionator, supplied by Biocomp Instruments Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/piston+gradient+fractionater/fractionator+gradient+piston/pmc12432361-436-21-24
Average 86 stars, based on 1 article reviews
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piston gradient fractionator biocomp  (Bio-Rad)
93
Bio-Rad piston gradient fractionator biocomp
Piston Gradient Fractionator Biocomp, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/piston+gradient+fractionater/Liquichek+Elevated+CRP+Control/pmc05580712__supp_gr__202150__115_Supplemental_Materials-10-7-26
Average 93 stars, based on 1 article reviews
piston gradient fractionator biocomp - by Bioz Stars, 2026-10
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piston gradient fractionator  (BioComp Systems Inc)
90
BioComp Systems Inc piston gradient fractionator
Piston Gradient Fractionator, supplied by BioComp Systems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/piston+gradient+fractionater/piston+gradient+fractionator/pmc01838968-106-51-54
Average 90 stars, based on 1 article reviews
piston gradient fractionator - by Bioz Stars, 2026-10
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piston gradient station  (Biocomp Instruments Inc)
86
Biocomp Instruments Inc piston gradient station
Piston Gradient Station, supplied by Biocomp Instruments Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/piston+gradient+fractionater/gradient+station/bio_rxiv__2025__08__29__670302-59-5-18
Average 86 stars, based on 1 article reviews
piston gradient station - by Bioz Stars, 2026-10
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gradient master  (New Brunswick Scientific)
86
New Brunswick Scientific gradient master
Gradient Master, supplied by New Brunswick Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/piston+gradient+fractionater/gradient+master/pmc08571055-510-1-10
Average 86 stars, based on 1 article reviews
gradient master - by Bioz Stars, 2026-10
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piston-driven gradient former  (Jule Inc)
90
Jule Inc piston-driven gradient former
Piston Driven Gradient Former, supplied by Jule Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/piston+gradient+fractionater/jule+gradient+former/pmc03201834-47-43-46
Average 90 stars, based on 1 article reviews
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fraction collector  (Bio-Rad)
94
Bio-Rad fraction collector
Fraction Collector, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/piston+gradient+fractionater/Model+2110+Fraction+Collector/bio_rxiv__2020__08__13__244970-209-16-20
Average 94 stars, based on 1 article reviews
fraction collector - by Bioz Stars, 2026-10
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◦ c  (Biocomp Instruments Inc)
86
Biocomp Instruments Inc ◦ c
◦ C, supplied by Biocomp Instruments Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/piston+gradient+fractionater/4%E2%97%A6c/pmc12432361__mmc4-601-8-24
Average 86 stars, based on 1 article reviews
◦ c - by Bioz Stars, 2026-10
86/100 stars
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optiprep  (Axis-Shield Diagnostics)
90
Axis-Shield Diagnostics optiprep
Sec12 is dispersed from the ER exit sites upon cTAGE5 knockdown. (A) HeLa cells were transfected with control, cTAGE5, or Sec12 siRNA. After 48 h, the cells were fixed with cold methanol and stained with Sec12, cTAGE5 CT, or Sec16 antibodies. (B) HeLa cells were transfected with control or cTAGE5 siRNA. After 48 h, cells were fixed with PFA and stained with Sec12 and Sec16 antibodies. The nuclei were stained with DAPI (blue). (right) Magnifications of the indicated regions on the left. (C) Quantification of Mander’s colocalization coefficient of B. n = 4. Error bars represent means ± SEM. **, P < 0.001. (D) HeLa cells were transfected with control or cTAGE5 siRNA. After 48 h, cells were fixed with PFA and stained with Sec12 and KDEL antibodies. The nuclei were stained with DAPI (blue). (right) Magnifications of the indicated regions on the left. (E) Quantification of Mander’s colocalization coefficient of D. n = 4. Error bars represent means ± SEM. *, P < 0.05. (F) HeLa cells transfected with siRNAs were extracted and subject to SDS-PAGE followed by Western blotting with TANGO1, cTAGE5 CC1, Sec12 (clone 7A10), and β-actin antibodies. For control knockdown, lysates were serially diluted. (G) HeLa cells were treated with control or cTAGE5 siRNA. After 48 h, HeLa cells were collected, and postnuclear supernatants were fractionated using a 0–26% <t>OptiPrep</t> density gradient. Fractions (fr.) were collected and analyzed by SDS-PAGE followed by Western blotting with EEA1, GM130, calnexin, and Sec12 (clone 7A10) antibodies. KD, knockdown. Bars, 10 µm.
Optiprep, supplied by Axis-Shield Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/piston+gradient+fractionater/optiprep/pmc04164946-195-6-7
Average 90 stars, based on 1 article reviews
optiprep - by Bioz Stars, 2026-10
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pharmacia lkb superfrac  (Pharmacia LKB Biotechnology Inc)
90
Pharmacia LKB Biotechnology Inc pharmacia lkb superfrac
Sec12 is dispersed from the ER exit sites upon cTAGE5 knockdown. (A) HeLa cells were transfected with control, cTAGE5, or Sec12 siRNA. After 48 h, the cells were fixed with cold methanol and stained with Sec12, cTAGE5 CT, or Sec16 antibodies. (B) HeLa cells were transfected with control or cTAGE5 siRNA. After 48 h, cells were fixed with PFA and stained with Sec12 and Sec16 antibodies. The nuclei were stained with DAPI (blue). (right) Magnifications of the indicated regions on the left. (C) Quantification of Mander’s colocalization coefficient of B. n = 4. Error bars represent means ± SEM. **, P < 0.001. (D) HeLa cells were transfected with control or cTAGE5 siRNA. After 48 h, cells were fixed with PFA and stained with Sec12 and KDEL antibodies. The nuclei were stained with DAPI (blue). (right) Magnifications of the indicated regions on the left. (E) Quantification of Mander’s colocalization coefficient of D. n = 4. Error bars represent means ± SEM. *, P < 0.05. (F) HeLa cells transfected with siRNAs were extracted and subject to SDS-PAGE followed by Western blotting with TANGO1, cTAGE5 CC1, Sec12 (clone 7A10), and β-actin antibodies. For control knockdown, lysates were serially diluted. (G) HeLa cells were treated with control or cTAGE5 siRNA. After 48 h, HeLa cells were collected, and postnuclear supernatants were fractionated using a 0–26% <t>OptiPrep</t> density gradient. Fractions (fr.) were collected and analyzed by SDS-PAGE followed by Western blotting with EEA1, GM130, calnexin, and Sec12 (clone 7A10) antibodies. KD, knockdown. Bars, 10 µm.
Pharmacia Lkb Superfrac, supplied by Pharmacia LKB Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/piston+gradient+fractionater/superfrac/pm33357414-812-10-10
Average 90 stars, based on 1 article reviews
pharmacia lkb superfrac - by Bioz Stars, 2026-10
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lkb 22238 uvicord sii uv detector  (Pharmacia LKB Biotechnology Inc)
90
Pharmacia LKB Biotechnology Inc lkb 22238 uvicord sii uv detector
Sec12 is dispersed from the ER exit sites upon cTAGE5 knockdown. (A) HeLa cells were transfected with control, cTAGE5, or Sec12 siRNA. After 48 h, the cells were fixed with cold methanol and stained with Sec12, cTAGE5 CT, or Sec16 antibodies. (B) HeLa cells were transfected with control or cTAGE5 siRNA. After 48 h, cells were fixed with PFA and stained with Sec12 and Sec16 antibodies. The nuclei were stained with DAPI (blue). (right) Magnifications of the indicated regions on the left. (C) Quantification of Mander’s colocalization coefficient of B. n = 4. Error bars represent means ± SEM. **, P < 0.001. (D) HeLa cells were transfected with control or cTAGE5 siRNA. After 48 h, cells were fixed with PFA and stained with Sec12 and KDEL antibodies. The nuclei were stained with DAPI (blue). (right) Magnifications of the indicated regions on the left. (E) Quantification of Mander’s colocalization coefficient of D. n = 4. Error bars represent means ± SEM. *, P < 0.05. (F) HeLa cells transfected with siRNAs were extracted and subject to SDS-PAGE followed by Western blotting with TANGO1, cTAGE5 CC1, Sec12 (clone 7A10), and β-actin antibodies. For control knockdown, lysates were serially diluted. (G) HeLa cells were treated with control or cTAGE5 siRNA. After 48 h, HeLa cells were collected, and postnuclear supernatants were fractionated using a 0–26% <t>OptiPrep</t> density gradient. Fractions (fr.) were collected and analyzed by SDS-PAGE followed by Western blotting with EEA1, GM130, calnexin, and Sec12 (clone 7A10) antibodies. KD, knockdown. Bars, 10 µm.
Lkb 22238 Uvicord Sii Uv Detector, supplied by Pharmacia LKB Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/piston+gradient+fractionater/uvicord+sii+2138+uv+detector/pm33357414-812-18-10
Average 90 stars, based on 1 article reviews
lkb 22238 uvicord sii uv detector - by Bioz Stars, 2026-10
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sw41 rotor  (Beckman Coulter)
96
Beckman Coulter sw41 rotor
Sec12 is dispersed from the ER exit sites upon cTAGE5 knockdown. (A) HeLa cells were transfected with control, cTAGE5, or Sec12 siRNA. After 48 h, the cells were fixed with cold methanol and stained with Sec12, cTAGE5 CT, or Sec16 antibodies. (B) HeLa cells were transfected with control or cTAGE5 siRNA. After 48 h, cells were fixed with PFA and stained with Sec12 and Sec16 antibodies. The nuclei were stained with DAPI (blue). (right) Magnifications of the indicated regions on the left. (C) Quantification of Mander’s colocalization coefficient of B. n = 4. Error bars represent means ± SEM. **, P < 0.001. (D) HeLa cells were transfected with control or cTAGE5 siRNA. After 48 h, cells were fixed with PFA and stained with Sec12 and KDEL antibodies. The nuclei were stained with DAPI (blue). (right) Magnifications of the indicated regions on the left. (E) Quantification of Mander’s colocalization coefficient of D. n = 4. Error bars represent means ± SEM. *, P < 0.05. (F) HeLa cells transfected with siRNAs were extracted and subject to SDS-PAGE followed by Western blotting with TANGO1, cTAGE5 CC1, Sec12 (clone 7A10), and β-actin antibodies. For control knockdown, lysates were serially diluted. (G) HeLa cells were treated with control or cTAGE5 siRNA. After 48 h, HeLa cells were collected, and postnuclear supernatants were fractionated using a 0–26% <t>OptiPrep</t> density gradient. Fractions (fr.) were collected and analyzed by SDS-PAGE followed by Western blotting with EEA1, GM130, calnexin, and Sec12 (clone 7A10) antibodies. KD, knockdown. Bars, 10 µm.
Sw41 Rotor, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/piston+gradient+fractionater/SW+41+Ti+Rotor/pm26934223-423-14-16
Average 96 stars, based on 1 article reviews
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Image Search Results


Sec12 is dispersed from the ER exit sites upon cTAGE5 knockdown. (A) HeLa cells were transfected with control, cTAGE5, or Sec12 siRNA. After 48 h, the cells were fixed with cold methanol and stained with Sec12, cTAGE5 CT, or Sec16 antibodies. (B) HeLa cells were transfected with control or cTAGE5 siRNA. After 48 h, cells were fixed with PFA and stained with Sec12 and Sec16 antibodies. The nuclei were stained with DAPI (blue). (right) Magnifications of the indicated regions on the left. (C) Quantification of Mander’s colocalization coefficient of B. n = 4. Error bars represent means ± SEM. **, P < 0.001. (D) HeLa cells were transfected with control or cTAGE5 siRNA. After 48 h, cells were fixed with PFA and stained with Sec12 and KDEL antibodies. The nuclei were stained with DAPI (blue). (right) Magnifications of the indicated regions on the left. (E) Quantification of Mander’s colocalization coefficient of D. n = 4. Error bars represent means ± SEM. *, P < 0.05. (F) HeLa cells transfected with siRNAs were extracted and subject to SDS-PAGE followed by Western blotting with TANGO1, cTAGE5 CC1, Sec12 (clone 7A10), and β-actin antibodies. For control knockdown, lysates were serially diluted. (G) HeLa cells were treated with control or cTAGE5 siRNA. After 48 h, HeLa cells were collected, and postnuclear supernatants were fractionated using a 0–26% OptiPrep density gradient. Fractions (fr.) were collected and analyzed by SDS-PAGE followed by Western blotting with EEA1, GM130, calnexin, and Sec12 (clone 7A10) antibodies. KD, knockdown. Bars, 10 µm.

Journal: The Journal of Cell Biology

Article Title: Concentration of Sec12 at ER exit sites via interaction with cTAGE5 is required for collagen export

doi: 10.1083/jcb.201312062

Figure Lengend Snippet: Sec12 is dispersed from the ER exit sites upon cTAGE5 knockdown. (A) HeLa cells were transfected with control, cTAGE5, or Sec12 siRNA. After 48 h, the cells were fixed with cold methanol and stained with Sec12, cTAGE5 CT, or Sec16 antibodies. (B) HeLa cells were transfected with control or cTAGE5 siRNA. After 48 h, cells were fixed with PFA and stained with Sec12 and Sec16 antibodies. The nuclei were stained with DAPI (blue). (right) Magnifications of the indicated regions on the left. (C) Quantification of Mander’s colocalization coefficient of B. n = 4. Error bars represent means ± SEM. **, P < 0.001. (D) HeLa cells were transfected with control or cTAGE5 siRNA. After 48 h, cells were fixed with PFA and stained with Sec12 and KDEL antibodies. The nuclei were stained with DAPI (blue). (right) Magnifications of the indicated regions on the left. (E) Quantification of Mander’s colocalization coefficient of D. n = 4. Error bars represent means ± SEM. *, P < 0.05. (F) HeLa cells transfected with siRNAs were extracted and subject to SDS-PAGE followed by Western blotting with TANGO1, cTAGE5 CC1, Sec12 (clone 7A10), and β-actin antibodies. For control knockdown, lysates were serially diluted. (G) HeLa cells were treated with control or cTAGE5 siRNA. After 48 h, HeLa cells were collected, and postnuclear supernatants were fractionated using a 0–26% OptiPrep density gradient. Fractions (fr.) were collected and analyzed by SDS-PAGE followed by Western blotting with EEA1, GM130, calnexin, and Sec12 (clone 7A10) antibodies. KD, knockdown. Bars, 10 µm.

Article Snippet: Supernatants were loaded onto the 0–26% OptiPrep (AXIS-SHIELD) density gradient and centrifuged with a rotor (MLS-50; Beckman Coulter) at 50,000 rpm for 2 h. Fractions were collected from top to bottom by a piston gradient fractionator (Biocomp Instruments).

Techniques: Transfection, Staining, SDS Page, Western Blot